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KMID : 1011420200250040405
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Journal of Korean Ophthalmic Optics Society
2020 Volume.25 No. 4 p.405 ~ p.411
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Localization of Nicotinic Acetylcholine Receptor α7 and β4 Subunits on Direction-Selective Ganglion Cells in Rabbit Retina
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Kwon Oh-Ju
Ahn Chang-Hyun
Jeon Chang-Jin
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Abstract
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Purpose: We investigated the existence and distribution of the nicotinic acetylcholine receptor (nAChR) α7 and β4 subunits on the dendritic arbors of direction-selective retinal ganglion cells (DSRGCs) in the adult rabbit retina.
Methods: DSRGCs were injected with 5% Lucifer yellow to identify their dendritic morphology. The nAChR α7 and β4 subunits on the dendritic arbors of the DSRGCs in the adult rabbit retina were identified using immunocytochemistry. The double-labeled images of dendrites and nAChR α7 and β4 subunits were visualized for reconstruction using high-resolution confocal microscopy.
Results: The distribution of nAChR α7 and β4 subunits on dendritic arbors in both the ON and OFF layers of the DSRGCs did not reveal an asymmetrical pattern in adult animals.
Conclusions: Although the present study suggests that the distributional pattern of the nAChR α7 and β4 subunits on the dendritic arbors of the DSRGCs was not asymmetric in the adult rabbit retina, the findings provide anatomical evidence of nAChR subunits for direction selectivity of cholinergic input.
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KEYWORD
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Nicotinic Acetylcholine Receptor, Direction Selectivity, Retinal Ganglion Cell, Immunocytochemistry, Cell Injection
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